Nucleosomes: The Next Generation of Cancer Biomarkers
A nucleosome, composed of histone proteins and the DNA wrapped around them, is the core unit of chromatin. The affect nucleosomes have on chromatin structure and gene regulation is dependent upon the identity of the histones that make up the nucleosome, as well as any modifications to these histone proteins. With a plethora of histone protein variants and post-translational modifications that can occur on their “histone tails”, there are a vast number of possibilities when it comes to the exact identiity of a nucleosome and its function. Recently, some of these variants and modifications have been found to associate with cancers. Furthermore, just the total amount of nucleosomes detected can also indicate a disease state or correlate with efficacy of therapy.
Recently, “liquid biopsies”, or blood draws from which you can examine biomarkers, have taken hold as an easy, less invasive method to monitor disease state and treatment efficacy. Nucleosomes, histones, and DNA can be shed into the blood during cancer due to high cellular turnover or in response to chemotherapy. Free-floating DNA (cfDNA and ctDNA) is an attractive biomarker, but has shown to be less stable than nucleosomes. Additionally, next generation sequencing of DNA is cumbersome and costly compared to a high-throughput ELISA assay to detect intact nucleosomes. Nucleosome-specific detection assays represent a new generation of biomarker tests: easy, fast, cost-effective, and compatible with stable proteins in liquid biopsy samples.
Histone H3.1
Active Motif’s Nu.Q H3.1 Assay Kit is designed for the detection of levels of Histone H3.1-containing, circulating cell-free nucleosomes (cf-nucleosomes) in human serum in a high-throughput format. Histone H3.1, or the canonical form of Histone H3, is deposited during DNA replication and possibly also during repair. Study of H3.1 has identified cancer-associated mutations and differing affinities for the histone acetyltransferase HAT1. To examine H3-containing nucleosome for all variants, use our Nu.Q H3 Assay. For added convenience and a more quantitative interpretation of results, the Nu.Q H3.1 Assay Kit also includes a recombinant nucleosome protein for use as a reference standard curve. For complete details, click the Nu.Q™ Method tab below.
Why use Nu.Q H3.1?
- Sensitivity: Detect circulating nucleosomes in as little as 10 µl of serum or 20 µl of plasma
- Specificity: Nucleosome epitope specific antibody enables detection of only intact nucleosomes
- Quantification: Recombinant nucleosomes enable relative quantification of levels of circulating H3.1 nucleosomes
- Convenience: Colorimetric assay in a simple 96-stripwell format for high and low throughput
- Fast: Results can be obtained in 5 hours.
More Active Motif Products
- CUT&Tag-IT Assay Kit CUT&Tag-IT Assay Kit Overview Cleavage Under Targets and Tagmentation (CUT&Tag) is a method to investigate genomic localization of histone modifications and some transcription factors …
- ATAC-Seq Kit Analyze open chromatin regions at genome-wide scale. ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or …
- PIXUL™ Multi-Sample Sonicator PIXUL™ Multi-Sample Sonicator Overview Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample …
- Low Cell ChIP-Seq Optimization Module Maximize retainment of limited ChIP-Seq samples When performing ChIP-Seq on limited samples, maximizing retainment of the already precious sample is key. Active Motif’s Low Cell Optimization Module …
- Nu.Q H3.1 Determine levels of H3.1-containing, circulating cell-free nucleosomes Active Motif’s Nu.Q™ H3.1 Assay Kit is designed for the detection of levels of Histone H3.1-containing, circulating …
 Elisa Assay Kit) Adiponectin (mouse) Elisa Assay Kit |
|||
| K4902-100 | Biovision | EUR 865 | |
 Elisa Assay Kit) Adiponectin (rat) Elisa Assay Kit |
|||
| K4903-100 | Biovision | EUR 843 | |
 Elisa Assay Kit) Visfatin (human) Elisa Assay Kit |
|||
| K4907-100 | Biovision | EUR 985 | |
 Elisa Assay Kit) Adiponectin (human) Elisa Assay Kit |
|||
| K2192-96 | ApexBio | 96 assays | EUR 1017 |
Adiponectin (mouse) Elisa Assay Kit |
|||
| K2193-96 | ApexBio | 96 assays | EUR 1017 |
) XTT CELL VIABILITY ASSAY KIT (1000 ASSAY) |
|||
| 30007 | Biotium | 1KIT | EUR 236 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
|||
) Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay) |
|||
| CBA-200 | Cell Biolabs | 50 assays | EUR 560 |
|
Description: For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor cells. These cells proliferate to form new blood vessels. Our Endothelial Tube Formation Assay (In Vitro Angiogenesis) provides an easy, robust system to assess angiogenesis in vitro. The ECM gel matrix very closely resembles an in vivo environment. |
|||
 Feline SAA Detection ELISA Assay Kit |
|||
| 6028 | Chondrex | 1 kit | EUR 553.15 |
|
Description: Feline SAA Detection ELISA Assay Kit |
|||
 Glycosaminoglycans Assay Kit |
|||
| 6022 | Chondrex | 1 kit | EUR 419.75 |
|
Description: Glycosaminoglycans Assay Kit |
|||
 DNA Assay Kit |
|||
| 6023 | Chondrex | 1 kit | EUR 180.5 |
|
Description: DNA Assay Kit |
|||
 Hemoglobin Assay Kit |
|||
| 6024 | Chondrex | 1 kit | EUR 180.5 |
|
Description: Hemoglobin Assay Kit |
|||
 Proteasome Assay Kit |
|||
| 55R-1341 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Proteasome in the research laboratory |
|||
 Calpain Assay Kit |
|||
| 55R-1342 | Fitzgerald | 100 assays | EUR 779 |
|
Description: Assay Kit for detection of Calpain in the research laboratory |
|||
 Glutathione Assay Kit |
|||
| 55R-1343 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Glutathione in the research laboratory |
|||
Glutathione Assay Kit |
|||
| 55R-1354 | Fitzgerald | 100 assays | EUR 895 |
|
Description: Assay Kit for detection of Glutathione activity in the research laboratory |
|||
 HDAC Assay Kit |
|||
| 55R-1371 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of HDAC in the research laboratory |
|||
HDAC Assay Kit |
|||
| 55R-1372 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of HDAC in the research laboratory |
|||
 HAT Assay Kit |
|||
| 55R-1373 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of HAT in the research laboratory |
|||
 SOD Assay Kit |
|||
| 55R-1374 | Fitzgerald | 100 assays | EUR 602 |
|
Description: Assay Kit for detection of SOD in the research laboratory |
|||
 HDAC3 Assay Kit |
|||
| 55R-1377 | Fitzgerald | 100 assays | EUR 693 |
|
Description: Assay Kit for detection of HDAC3 in the research laboratory |
|||
 HDAC8 Assay Kit |
|||
| 55R-1379 | Fitzgerald | 100 assays | EUR 689 |
|
Description: Assay Kit for detection of HDAC8 in the research laboratory |
|||
 ATP Assay Kit |
|||
| 55R-1380 | Fitzgerald | 100 assays | EUR 809 |
|
Description: Assay Kit for detection of ATP in the research laboratory |
|||
 ADP Assay Kit |
|||
| 55R-1381 | Fitzgerald | 100 assays | EUR 809 |
|
Description: Assay Kit for detection of ADP in the research laboratory |
|||
 FAD Assay Kit |
|||
| 55R-1382 | Fitzgerald | 100 assays | EUR 732 |
|
Description: Assay Kit for detection of FAD in the research laboratory |
|||
 PEP Assay Kit |
|||
| 55R-1384 | Fitzgerald | 100 assays | EUR 876 |
|
Description: Assay Kit for detection of PEP in the research laboratory |
|||
 Ammonia Assay Kit |
|||
| 55R-1388 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Ammonia in the research laboratory |
|||
 cAMP Assay Kit |
|||
| 55R-1389 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of cAMP activity in the research laboratory |
|||
 cGMP Assay Kit |
|||
| 55R-1390 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of cGMP activity in the research laboratory |
|||
 Urea Assay Kit |
|||
| 55R-1391 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Urea in the research laboratory |
|||
 Calcium Assay Kit |
|||
| 55R-1392 | Fitzgerald | 250 assays | EUR 586 |
|
Description: Assay Kit for detection of Calcium activity in the research laboratory |
|||
 Magnesium Assay Kit |
|||
| 55R-1393 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Magnesium in the research laboratory |
|||
 Zinc Assay Kit |
|||
| 55R-1394 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of Zinc in the research laboratory |
|||
 Iron Assay Kit |
|||
| 55R-1395 | Fitzgerald | 100 assays | EUR 758 |
|
Description: Assay Kit for detection of Iron in the research laboratory |
|||
 Phosphate Assay Kit |
|||
| 55R-1400 | Fitzgerald | 500 assays | EUR 415 |
|
Description: Assay Kit for detection of Phosphate in the research laboratory |
|||
 JNK Assay Kit |
|||
| 55R-1407 | Fitzgerald | 40 assays | EUR 868 |
|
Description: Assay Kit for detection of JNK in the research laboratory |
|||
JNK Assay Kit |
|||
| 55R-1408 | Fitzgerald | 40 assays | EUR 979 |
|
Description: Assay Kit for detection of JNK in the research laboratory |
|||
 Akt Assay Kit |
|||
| 55R-1409 | Fitzgerald | 40 assays | EUR 979 |
|
Description: Assay Kit for detection of Akt in the research laboratory |
|||
Ammonia Assay Kit |
|||
| 55R-1410 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Ammonia in the research laboratory |
|||
 Cobalt Assay Kit |
|||
| 55R-1435 | Fitzgerald | 100 assays | EUR 621 |
|
Description: Assay Kit for detection of Cobalt in the research laboratory |
|||