Ready-Mix kit contains all necessary reagents (excluding the template and primers) for efficient, optimized, specific and sensitive end-point PCR reaction. The PCR product can immediately be analyzed by an Agarose-gel electrophoresis and also could be purified by using PCR purification kit.
Bio-ReadyMix Start (hot start) kit contains Hot-Start Taq polymerase to enable more specificity, accuracy and sensitivity of PCR reaction.
Rapid H&E stain kit
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2× reaction mix with Hot-Start Taq DNA polymerase for conventional PCR, contains dyes for analysis of PCR products by gel electrophoresis
Conventional PCR with high reproducibility
Generation of PCR products for TA cloning
RT-PCR (two-step method)
BioMaster HS-Taq PCR-Color (2×) kit contains 2× BioMaster HS-Taq PCR-Color reaction mix, 50 mM MgCl2 and sterile water. BioMaster HS-Taq PCR-Color (2×) reaction mix has been developed for \ PCR analysis of a large number of samples. BioMaster HS-Taq PCR-Color (2×) reaction mix includes all of the components (except for DNA template and primers) necessary to carry out PCR: highly processive recombinant HS-Taq DNA polymerase, deoxynucleoside triphosphate mixture, 2× PCR buffer, Mg2+ and marker dyes.
The mix is optimized for efficient and reproducible hot-start PCR. The master mix is supplemented with additives that increase the half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster HS-Taq PCR-Color (2×) reaction mix is chemically stable, inert and does not interfere with optimal annealing temperature or the parameters of template melting. The presented DNA polymerase is inactive at room temperature; preheating of reaction solution at 95 °C for 5 min is required for enzyme activation. Additional solution of MgCl2 allows easy optimization for each individual primer/template system. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps. The included dyes and increased density of the solution allow loading the reaction mix directly on gel for electrophoresis.
The 96-well Plasmid Kit is designed for rapid high throughput isolation of plasmid or cosmid DNA from 1- 2 ml of bacterial cultures. All components and reagents are provided for a complete solution. In the process, the modified alkaline lysis method and RNase A treatment are used to obtain cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the Plasmid Binding Plate. The contaminants are washed off with an ethanol-based wash buffer and finally, the purified plasmid DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. Typical yields are 5-10 μg for high-copy number plasmid and 0.5-5μg for low-copy number plasmids. The entire procedure can be completed within 60 minutes and the purified plasmid DNA is ready for restiction digestion, ligation, PCR, and sequencing reaction.
Description: Cytokine storm syndromes is an activation cascade of pro-inflammatory cytokines，such as TNF-α，IL-1，IL-6，IL-12，IFN-α，IFN-β，IFN-γ，MCP-1 and IL-8,due to unregulated host immune response system to different stimuli such as infections, malignancy, rheumatoid disorders, drugs, and so on. Interferon-γ (IFN-γ), one of the four subclasses ofinterferons, and is a multipotent protein, acting on many cell types by inducing or inhibiting many cellular functions through direct effects on gene expression. This ELISA assay pair contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IFN-γ.
Description: Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. This ELISA assay pair contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TNF-α.
PCSK9 [Biotinylated] : LDL R Inhibitor Screening ELISA Assay Pair
Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Description: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.