Product details
Applications
- Western Blot (WB)
- ELISA (ELISA)
Proven dilution
- Trial dependent
- Trial dependent
Product specifications
Species reactivity: Dog
Host / isotype: Mouse / IgG2a
Class: Monoclonal
Writes: Secondary antibody
Clone: E6-2A1
Immunogen
Preparation of affinity-purified IgE from highly parasitized and allergic dog serum.
Conjugate: Unconjugated
Form: Liquid
Concentration: 1 mg / mL
Purification: Protein A
Storage buffer: PBS
Contains: <0.1% sodium azide
Storage conditions
4 ° C or -20 ° C if preferred
RRID: AB_2538146
Objective: IgE
Form of antibody: Whole Antibody
Product-specific information
This antibody recognizes canine IgE and does not cross-react with canine IgM, IgA, IgG1, or IgG2. Western blot analysis against affinity-purified IgE shows a single 62-65 kDa major band under reducing conditions.
Destination information
Thermo Scientific Anti-Canine Secondary Antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins and are useful in the detection, classification, or purification of their specific target. Secondary antibodies offer greater versatility allowing users to use many detection systems (eg HRP, AP, fluorescence).
They can also provide increased sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. More commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e., immunoaffinity chromatography, antibody fragmentation, marker conjugation, etc.) to generate highly specific reagents.