Product details


  • Western Blot (WB)

Proven dilution

  • Trial dependent
  • Trial dependent

Product specifications

Species reactivity: Dog

Host / isotype: Mouse / IgG2a

Class: Monoclonal

Writes: Secondary antibody

Clone: E6-2A1


Preparation of affinity-purified IgE from highly parasitized and allergic dog serum.

Conjugate: Unconjugated

Form: Liquid

Concentration: 1 mg / mL

Purification: Protein A

Storage buffer: PBS

Contains: <0.1% sodium azide

Storage conditions

4 ° C or -20 ° C if preferred

RRID: AB_2538146

Objective: IgE

Form of antibody: Whole Antibody

Product-specific information

This antibody recognizes canine IgE and does not cross-react with canine IgM, IgA, IgG1, or IgG2. Western blot analysis against affinity-purified IgE shows a single 62-65 kDa major band under reducing conditions.

Destination information

Thermo Scientific Anti-Canine Secondary Antibodies are affinity-purified antibodies with well-characterized specificity for canine (dog) immunoglobulins and are useful in the detection, classification, or purification of their specific target. Secondary antibodies offer greater versatility allowing users to use many detection systems (eg HRP, AP, fluorescence).

They can also provide increased sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. More commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e., immunoaffinity chromatography, antibody fragmentation, marker conjugation, etc.) to generate highly specific reagents.

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